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Background  Traditional diagnostic technology with tumor biomarkers is inefficient, expensive and requires a large number of serum samples. The purpose of this study was to construct human lung cancer protein chips with new lung cancer biomarkers screened by the  T 7‐phage display library, and improve the early diagnosis rate of lung cancer.    Methods  A  T 7‐phage  cDNA  display library was constructed of fresh samples from 30 lung cancer patients. With biopanning and high‐throughput screening, we gained the immunogenic phage clones from the  cDNA  library. The insert of selected phage was blasted at  GeneBank  for alignment to find the exact or the most similar known genes. Protein chips were then constructed and used to assay their expression level in lung cancer serum from 217 cases of lung cancer groups:80 cases of benign lung disease and 220 healthy controls.    Results  After four rounds of  B iopanning and two rounds of enzyme‐linked immunosorbent assay, 12 phage monoclonal samples were selected from 2880 phage monoclonal samples. After blasting at  GeneBank , six similar genes were used to construct diagnostic protein chips. The protein chips were then used to assay expression level in lung cancer serum. The expression level of six genes in lung cancer groups was significantly higher than those in the other two groups ( P  < 0.05).    Conclusions  In this study, we successfully constructed diagnostic protein chips with biomarkers selected from the lung cancer  T 7‐phage  cDNA  library, which can be used for the early screening of lung cancer patients.

Diagnostic value of protein chips constructed by lung‐cancer‐associated markers selected by the T 7 phage display library