Open AccessOne‐pot synthesis of 6‐aminohexanoic acid from cyclohexane using mixed‐species cultures
Author(s) -
Bretschneider Lisa,
Wegner Martin,
Bühler Katja,
Bühler Bruno,
Karande Rohan
Publication year - 2021
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13744
Subject(s) - cyclohexane , yield (engineering) , chemistry , substrate (aquarium) , downstream processing , bioprocess , cascade reaction , combinatorial chemistry , organic chemistry , materials science , chemical engineering , biochemistry , catalysis , biology , ecology , metallurgy , engineering
A 6‐step cascade is distributed among P. taiwanensis and E. coli to synthesize the Nylon‐6 monomer 6‐aminohexanoic acid from cyclohexane under environmentally friendly conditions. The appropriate shuttle compound, avoidance of substrate toxicity, and mass transfer considerations finally enabled 86% 6‐aminohexanoic acid yield in a mixed‐species biotransformation.