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Using the Latour system, a hybrid NRPS‐T1PKS cluster (˜20 kb) which was responsible for phthoxazolin biosynthesis was efficiently deleted in  S. pogona . A TetR family transcriptional regulatory gene that could regulate the butenyl‐spinosyn biosynthesis has been identified from the phthoxazolin biosynthetic gene cluster.

microbial biotechnology

Deletion of a hybrid NRPS‐T1PKS biosynthetic gene cluster via Latour gene knockout system in  Saccharopolyspora pogona  and its effect on butenyl‐spinosyn biosynthesis and growth development