Open AccessDeletion of a hybrid NRPS‐T1PKS biosynthetic gene cluster via Latour gene knockout system in Saccharopolyspora pogona and its effect on butenyl‐spinosyn biosynthesis and growth development
Author(s) -
Rang Jie,
Li Yunlong,
Cao Li,
Shuai Ling,
Liu Yang,
He Haocheng,
Wan Qianqian,
Luo Yuewen,
Yu Ziquan,
Zhang Youming,
Sun Yunjun,
Ding Xuezhi,
Hu Shengbiao,
Xie Qingji,
Xia Liqiu
Publication year - 2021
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13694
Subject(s) - gene cluster , biology , gene , biosynthesis , genome , genetics , mutant , secondary metabolism
Using the Latour system, a hybrid NRPS‐T1PKS cluster (˜20 kb) which was responsible for phthoxazolin biosynthesis was efficiently deleted in S. pogona . A TetR family transcriptional regulatory gene that could regulate the butenyl‐spinosyn biosynthesis has been identified from the phthoxazolin biosynthetic gene cluster.