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Liquid superficial‐static culture was a better method to produce GA. Transcriptome dynamics analysis revealed CYP5150L8 was the key gene regulating lanosterol flux into GA biosynthesis. Other sixteen CYP450s genes were significantly higher‐expressed during the culture time in LSSC and could be potential candidate genes associated with the biosynthesis of different GAs.

microbial biotechnology

Transcriptome dynamics and metabolite analysis revealed the candidate genes and regulatory mechanism of ganoderic acid biosynthesis during liquid superficial‐static culture of  Ganoderma lucidum