Open AccessAn innovative protein expression system using RNA polymerase I for large‐scale screening of high‐nucleic‐acid content Saccharomyces cerevisiae strains
Author(s) -
Zeng Duwen,
Qiu Chenxi,
Shen Yu,
Hou Jin,
Li Zailu,
Zhang Jixiang,
Liu Shuai,
Shang Jianli,
Qin Wensheng,
Xu Lili,
Bao Xiaoming
Publication year - 2020
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13653
Subject(s) - biology , rna polymerase iii , ura3 , saccharomyces cerevisiae , rna , rna polymerase i , terminator (solar) , ribosomal rna , nucleic acid , gene , microbiology and biotechnology , rna polymerase , genetics , ionosphere , physics , astronomy
By using the more effective IRES (internal ribosome entry site) element, an innovative cap‐independent protein expression system mediated by RNA polymerase I, which only transcribes rRNA normally, was established in Saccharomyces cerevisiae . Such system made the reporter gene yEGFP3 express variation especially after mutagenesis. Combined with flow cytometry, cells with high fluorescence intensity were sorted from 200,000 cells and the strain with 58% RNA content higher than the parental strain was obtained. So the model for large‐scale screening of high‐nucleic‐acid content yeasts was set up.