Open AccessFirst description of betalains biosynthesis in an aquatic organism: characterization of 4,5‐DOPA‐extradiol‐dioxygenase activity in the cyanobacteria Anabaena cylindrica
Author(s) -
GuerreroRubio María Alejandra,
GarcíaCarmona Francisco,
GandíaHerrero Fernando
Publication year - 2020
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13641
Subject(s) - enzyme , cyanobacteria , biochemistry , biosynthesis , biology , dioxygenase , anabaena , betalain , heterologous expression , bacteria , chemistry , gene , pigment , genetics , organic chemistry , recombinant dna
This paper describes molecular and functional characterization of a novel dioxygenase enzyme from the aquatic cyanobacterium Anabaena cylindrica . The enzyme showed a strong inhibition by excess of the precursor L‐DOPA. However, its heterologous expression has allowed detecting the formation of the main compounds in the biosynthetic pathway of betalains. The discovery of this novel dioxygenase in the phylum cyanobacteria shows that betalains' formation is more diverse than expected.