Open AccessLost in translation: the quest for Nitrosomonas cluster 7‐specific amoA primers and TaqMan probes
Author(s) -
Orschler Laura,
Agrawal Shelesh,
Lackner Susanne
Publication year - 2020
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13627
Subject(s) - primer (cosmetics) , taqman , nitrosomonas europaea , biology , nitrosomonas , computational biology , polymerase chain reaction , genetics , pyrosequencing , taq polymerase , dna , microbiology and biotechnology , gene , chemistry , ecology , thermus aquaticus , nitrite , organic chemistry , nitrate
This paper highlights the current challenges with quantitative estimation of ammonia‐oxidizing bacteria (AOB) employing quantitative real‐time PCR (qPCR) in diverse natural‐ and engineered‐ environments. In this work we concentrates on: (i) identifying the coverage and specificity of the available primers and TaqMan probes for amoA gene based on current databases; (ii) the development of new primers and TaqMan probes for differential quantification of N. europaea and N. eutropha .