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Efficient isolation of human metapneumovirus using MNT‐1, a human malignant melanoma cell line with early and distinct cytopathic effects
Author(s) -
Sato Ko,
Watanabe Oshi,
Ohmiya Suguru,
Chiba Fumiko,
Suzuki Akira,
Okamoto Michiko,
Younghuang Jiang,
Hata Akihiro,
aka Hiroyuki,
Kitaoka Setsuko,
Nagai Yukio,
Kawamura Kazuhisa,
Hayashi Masahiro,
Kumaki Satoru,
Suzuki Tamio,
Kawakami Kazuyoshi,
Nishimura Hidekazu
Publication year - 2017
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12542
Subject(s) - human metapneumovirus , metapneumovirus , vero cell , syncytium , virology , cell culture , biology , cytopathic effect , isolation (microbiology) , melanoma , virus , microbiology and biotechnology , cancer research , respiratory tract infections , respiratory system , genetics , anatomy
Isolation of human metapneumovirus (HMPV) from clinical specimens is currently inefficient because of the lack of a cell culture system in which a distinct cytopathic effect (CPE) occurs. The cell lines LLC‐MK2, Vero and Vero E6 are used for isolation of HMPV; however, the CPE in these cell lines is subtle and usually requires a long observation period and sometimes blind passages. Thus, a cell line in which an early and distinct CPE occurs following HMPV inoculation is highly desired by clinical virology laboratories. In this study, it was demonstrated that, in the human malignant melanoma cell line MNT‐1, obvious syncytium formation occurs shortly after inoculation with HMPV‐positive clinical specimens. In addition, the growth and efficiency of isolation of HMPV were greater using MNT‐1 than using any other conventional cell line. Addition of this cell line to our routine viral isolation system for clinical specimens markedly enhanced isolation frequency, allowing isolation‐based surveillance. MNT‐1 has the potential to facilitate clinical and epidemiological studies of HMPV.