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The rise of neutron cryo‐crystallography
Author(s) -
Kwon Hanna,
Langan Patricia S.,
Coates Leighton,
Raven Emma L.,
Moody Peter C. E.
Publication year - 2018
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.374
H-Index - 138
ISSN - 2059-7983
DOI - 10.1107/s205979831800640x
Subject(s) - neutron , crystallography , extrapolation , neutron diffraction , scope (computer science) , materials science , physics , computer science , nuclear physics , chemistry , crystal structure , mathematics , mathematical analysis , programming language
The use of boiled‐off liquid nitrogen to maintain protein crystals at 100 K during X‐ray data collection has become almost universal. Applying this to neutron protein crystallography offers the opportunity to significantly broaden the scope of biochemical problems that can be addressed, although care must be taken in assuming that direct extrapolation to room temperature is always valid. Here, the history to date of neutron protein cryo‐crystallography and the particular problems and solutions associated with the mounting and cryocooling of the larger crystals needed for neutron crystallography are reviewed. Finally, the outlook for further cryogenic neutron studies using existing and future neutron instrumentation is discussed.