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Greatly reduced radiation damage in ribonuclease crystals mounted on glass fibers
Author(s) -
Dewan J. C.,
Tilton R. F.
Publication year - 1987
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s0021889887087004
Subject(s) - diffractometer , quartz , radiation damage , crystal (programming language) , radiation , materials science , epoxy , composite material , glass fiber , irradiation , capillary action , optics , scanning electron microscope , physics , computer science , programming language , nuclear physics
X‐ray data have been collected from crystals of bovine pancreatic ribonuclease A at temperatures of 220K and below, by use of a nitrogen‐gas cold stream and a computer‐controlled diffractometer. One data set, collected from a crystal mounted in the traditional manner in a quartz capillary tube, showed radiation damage of 21% after 14.6 days of exposure to Cu Kα radiation at 160K. Another crystal, mounted on a glass fiber by use of uncured epoxy cement, showed only a 3% decay of the intensity standards after 28.3 days of exposure to the same radiation and at similar temperatures.