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A fibre‐based crystal mounting technique for protein cryocrystallography
Author(s) -
Sugahara Michihiro
Publication year - 2012
Publication title -
journal of applied crystallography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.429
H-Index - 162
ISSN - 1600-5767
DOI - 10.1107/s002188981200756x
Subject(s) - mosaicity , crystal (programming language) , zeolite , materials science , dispersion (optics) , protein crystallization , polyester , wavelength , crystallization , crystallography , optics , composite material , chemistry , optoelectronics , catalysis , epitaxy , computer science , organic chemistry , physics , layer (electronics) , programming language
The CryoFibre, a crystal mounting tool, has been developed for protein cryocrystallography. The technique attaches single crystals to the tips of polyester fibres, allowing removal of excess liquid around each crystal. Single‐wavelength anomalous dispersion phasing using a Cu K α X‐ray source (Cu SAD) was applied to crystals from five proteins without any derivatization, demonstrating a clear improvement in the success rate of Cu SAD compared with the conventional loop technique. In addition, a xylanase crystal on the surface of a synthetic zeolite as a hetero‐epitaxic nucleant was directly mounted on the CryoFibre without separation treatment of the crystal from the zeolite. The crystal had a lower mosaicity than that observed using the conventional technique, indicating that the fibre technique is suitable for high‐quality data collection from zeolite‐mediated crystals.