Open AccessLimnoglobus roseus gen. nov., sp. nov., a novel freshwater planctomycete with a giant genome from the family Gemmataceae
Author(s) -
Irina S. Kulichevskaya,
D. G. Naumoff,
Kirill K. Miroshnikov,
Anastasia A. Ivanova,
Dmitriy Philippov,
Աննա Հակոբյան,
W. Irene C. Rijpstra,
Jaap S. Sinninghe Damsté,
Werner Liesack,
Svetla. Dedysh
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003904
Subject(s) - biology , planctomycetes , 16s ribosomal rna , gene , glycoside hydrolase , genome , botany , genome size , genetics , microbiology and biotechnology , actinobacteria
The family Gemmataceae accommodates aerobic, chemoorganotrophic planctomycetes, which inhabit various freshwater ecosystems, wetlands and soils. Here, we describe a novel member of this family, strain PX52 T , which was isolated from a boreal eutrophic lake in Northern Russia. This isolate formed pink-pigmented colonies and was represented by spherical cells that occurred singly, in pairs or aggregates and multiplied by budding. Daughter cells were highly motile. PX52 T was an obligate aerobic chemoorganotroph, which utilized various sugars and some heteropolysaccharides. Growth occurred at pH 5.0–7.5 (optimum pH 6.5) and at temperatures between 10 and 30 °C (optimum 20–25 °C). The major fatty acids were C 18 : 1 ɷ7c, C 18 : 0 and βOH-C 16:0 ; the major intact polar lipid was trimethylornithine, and the quinone was MK-6. The complete genome of PX52 T was 9.38 Mb in size and contained nearly 8000 potential protein-coding genes. Among those were genes encoding a wide repertoire of carbohydrate-active enzymes (CAZymes) including 33 glycoside hydrolases (GH) and 87 glycosyltransferases (GT) affiliated with 17 and 12 CAZy families, respectively. DNA G+C content was 65.6 mol%. PX52 T displayed only 86.0–89.8 % 16S rRNA gene sequence similarity to taxonomically described Gemmataceae planctomycetes and differed from them by a number of phenotypic characteristics and by fatty acid composition. We, therefore, propose to classify it as representing a novel genus and species, Limnoglobus roseus gen. nov., sp. nov. The type strain is strain PX52 T (=KCTC 72397 T =VKM B-3275 T ).