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In vitro Evaluation of Ayurveda Herbs ( Centella asiatica, Bacopa monnieri, Rhizoma polygonata ) for Potential Carbohydrate Hydrolyzing Enzyme Inhibition
Author(s) -
Fortier Kevin,
Racicot Kenneth,
Apostolidis Emmanouil
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.924.25
Subject(s) - centella , bacopa monnieri , traditional medicine , chemistry , alpha glucosidase , sucrase , fractionation , inhibitory postsynaptic potential , ic50 , in vitro , food science , enzyme , biochemistry , chromatography , medicine
Three Ayurveda herbs ( C. asiatica / Gotu Kola, B. monnieri / Brahmi and R. polygonata / Fo‐Ti) were extracted in water and evaluated for their phenolic content and alpha‐glucosidase inhibitory activity. The phenolic content was evaluated using the Folin‐Ciocalteau's Assay and we observed that Fo‐Ti had the highest phenolic content (1.45 mg/mL GAEq), followed by Gotu Kola (0.41 mg/mL GAEq) and Brahmi (0.31 mg/mL GAEq). When we evaluated the dose‐dependent inhibitory activity of the three extracts against alpha‐glucosidase, Fo‐Ti had the highest inhibitory activity (55%). Gotu Kola and Brahmi had similar and very low inhibitory activities (13% and 22%, respectively). These results are expected since previous reports had shown that alpha‐glucosidase inhibitory activity is phenolic dependent and Fo‐Ti had significantly higher phenolic content, when compared to the other two tested samples. Currently, LH‐20 fractionation of Fo‐Ti is underway to identify the possible bioactive fractions responsible for the observed activity. Fo‐Ti and the resulting fractions will be evaluated for their specific sucrase and maltase inhibitory activity. Completion of this research will provide a better understanding of the possible mechanism of action of Fo‐Ti for potential type 2 diabetes prevention.