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Association of IDH1 Gene R132H Mutation and Increased Tumor HIF1‐α and Serum VEGF Levels in Primary Glioblastoma Multiforme
Author(s) -
Aydin Hikmet,
Yalaza Cem,
Ak Handan,
Cagli Mehmet,
Ozgiray Erkin,
Atay Sevcan
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.725.18
Subject(s) - idh1 , isocitrate dehydrogenase , carcinogenesis , brain tumor , glioblastoma , cancer research , primary tumor , medicine , mutation , pathology , cancer , biology , oncology , gene , genetics , metastasis , enzyme , biochemistry
Glioblastoma multiforme (GBM) is the most common form of primary brain tumors. Although mutations in isocitrate dehydrogenase‐1 ( IDH1 ) have been identified in a number of cancers, their role in tumor development has not been fully elucidated. In this preliminary study we aimed to investigate the association between IDH1 mutations, tumor tissue HIF‐1 alpha and serum VEGF levels in patients with primary GBM. 32 patients (mean age, years: 58 ± 14.0) diagnosed with primary glioblastoma multiforme were screened for IDH1 mutations (R132H, R132S, R132C and R132L) by direct sequencing. Serum VEGF and tumor tissue HIF1‐alpha levels were measured by ELISA. Associations between categoric variables were determined using chi‐square tests. Differences between two groups were compared with t test for continuous variables. Two patients were heterozygous for R132H mutation (6%). Tumor HIF1‐alpha and serum VEGF levels were found to be significantly increased in IDH1 ‐mutated tumor tissues.Wild Type IDH1 (n=30) Mutant IDH1 (n=2)pSerum VEGF (ng/ml) 0.189 ± 0.08 0.309 ± 0.016 0.0454Tumor HIF1‐α (ng/ml) 0.710 ± 0.835 5.058 ± 3.75 < 0.0001Our preliminary results suggest that mutated IDH1 may contributes to carcinogenesis via induction of HIF‐1 alpha pathway in primary GBM.