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Membrane Proteins ‐ the Lipid Connection
Author(s) -
Robinson Dame Carol
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.493.1
Subject(s) - membrane , membrane protein , membrane lipids , chemistry , peripheral membrane protein , function (biology) , mass spectrometry , biophysics , integral membrane protein , biochemistry , microbiology and biotechnology , chromatography , biology
Lipids are critical components of membranes and yet their precise composition and effects on membrane‐embedded protein complexes are often difficult to define. Using mass spectrometry I will show how we can add lipids and quantify their effects on the stability of membrane protein complexes using unfolding experiments. I will also show how results from these experiments inform about suitable solution conditions for crystallography and function. Turning to the endogenous lipid cohort that surrounds a membrane protein I will show how we can define these lipids and demonstrate their importance for function.