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Activation of Autophagy by Globular Adiponectin Attenuates Ethanol‐induced Apoptosis in HepG2 Cells
Author(s) -
Nepal Saroj,
Kim Mi Jin,
Subedi Amit,
Park PilHoon
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1106.9
Subject(s) - autophagy , apoptosis , microbiology and biotechnology , chemistry , programmed cell death , autophagosome , ethanol , biology , biochemistry
The present study was undertaken to investigate the role of autophagy induction by globular adiponectin (gAcrp) in the suppression of ethanol‐induced apoptosis and underlying mechanism(s) therein in human hepatoma cell lines (HepG2). Here, we demonstrated that gAcrp significantly restores ethanol‐induced suppression of autophagy‐related genes, including beclin‐1 and microtubule‐associated protein light chain (LC3B), and autophagosome formation in HepG2 cells. Furthermore, inhibition of gAcrp‐induced autophagic process by knock‐down of LC3B prevented protection from ethanol‐induced apoptosis. In particular, the autophagic process induced by gAcrp was involved in the suppression of ethanol‐induced activation of caspase‐8 and expression of Bax, indicating that autophagy induction by gAcrp plays a critical role in the prevention of ethanol‐induced apoptosis. Taken together, our data demonstrated that gAcrp protects liver cells from ethanol‐induced apoptosis via induction of autophagy.