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Amiloride Derivative Phenamil Restricts Long Bone Growth in Broilers in Conjunction with Zinc Accumulation
Author(s) -
Price Tara,
Moncada Kristin L,
Park Kye Won,
Tontonoz Peter,
Walzem Rosemary L.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1084.1
Subject(s) - chemistry , femur , broiler , tibia , endocrinology , medicine , bone ash , zinc , bone growth , zoology , body weight , anatomy , calcium , biology , surgery , food science , organic chemistry
Phenamil induces osteoblast formation and calcification in murine mesenchymal cultures. In vivo bioactivity in broiler chicks was studied during rapid bone growth 2 weeks post hatch. 26 broiler chicks were intubated daily with DMSO Vehicle (V) or 30 mg Phenamil (P)/kg BW. Body weight was measured to day 15 in ad lib fed birds. Tibia & femur fresh ( f ) and dry ( d ) weight, length, mid‐shaft width, % ash and mineral composition were measured. P‐birds weighed less than CON on days 8 through 14 (p< 0.03), but not at day 15 (p< 0.28). Tibia f and femur f weights from P‐birds were less than CON (p< 0.005, p< 0.002), but only tibia d weight as %BW differed (p< 0.04). Femur d weights did not differ. Tibias d from P‐birds weighed less than CON (p< 0.011), but not as a %BW. Bone ash as % of BW did not differ. Tibias f from P birds were shorter (p<0.004) & more narrow (p< 0.012) than CON. Femur lengths and widths were similar as was dry bone, bone ash %. Bone ppm of P, K, Ca, Mg, Na, Fe, Cu, Mn, S, and B were similar, while bone Zn increased 30% in P‐birds (p< 0.001) along with a 2.6% increase in bone Ca:P (p< 0.003). Phenamil restricted long bone growth of young broilers while enhancing relative bone Ca and absolute bone Zn deposition. Phenamil effects on normal bone growth as compared to bone repair need additional study. Supported in part by Undergraduate Research funds to KM. Animal Protocol #2012–028 IAUCC‐TAMU