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Association between the plasma proteome and plasma alphatocopherol concentrations in humans
Author(s) -
Da Costa Laura Anne,
García-Bailo Bibiana,
Borchers Christoph H,
Badawi Alaa,
El-Sohemy Ahmed
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1017.10
Subject(s) - proteome , medicine , blood proteins , vitamin e , fibrinogen , glycoprotein , alpha tocopherol , endocrinology , chemistry , biology , physiology , biochemistry , antioxidant
Vitamin E has been inversely associated with chronic disease; however, the biological processes regulated by this vitamin have not been fully elucidated. The objective of the present study was to examine the association between α‐tocopherol and the plasma proteome. Subjects are from the Toronto Nutrigenomics and Health Study and include men and women (n=1,022) who completed a general health and lifestyle questionnaire and 196‐ item food frequency questionnaire and provided a fasting blood sample. Levels of α‐tocopherol were analyzed using a reversed‐phase isocratic HPLC method with fluorescent detection. Fifty‐four plasma proteins were assayed by a mass spectrometry‐based multiple reaction monitoring method. Analysis of covariance was used to compare mean concentrations of plasma proteins across tertiles of α‐tocopherol. Levels of apolipoprotein C‐III, fibrinogen alpha, beta, and gamma chains, fibronectin and fibrinopeptide A were significantly and positively associated to plasma α‐tocopherol, while intermediate levels of α‐tocopherol were significantly associated with higher levels of alpha‐1B‐glycoprotein (all P<0.0009). In conclusion, circulating levels of α‐tocopherol were significantly associated with plasma proteins from several physiological pathways. This research was funded by the Advanced Foods and Materials Network.