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Kidney microRNA expression profile in Ang II‐dependent Hypertension
Author(s) -
GonzalezVillalobos Romer A,
Kim Catherine,
Neff Ryan M,
Satou Ryousuke,
Navar L. Gabriel
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.605.13
Subject(s) - microrna , medicine , blood pressure , kidney , endocrinology , angiotensin ii , renin–angiotensin system , microarray , gene expression , chemistry , gene , biochemistry
This study was conducted to determine if there are changes in microRNAs expression in the kidneys during angiotensin (Ang)‐II dependent hypertension. microRNAs are small non‐coding RNAs that cause posttranscriptional repression of their targets with emerging recognition as key players in kidney disease. C57BL/6J male mice (8–9 week) were subjected to sham operation (n=3) or Ang II infusions (400 ng/kg/min, n=3) for 12 days during which systolic blood pressure (SBP) was monitored by tail‐cuff method. RNA was isolated from the kidneys and microRNA expression was determined by microarray. SBP was increased by Ang II (151±3 mmHg) vs. controls (119±0.2, p<0.05). When compared to controls, the expression of 5 microRNAs was reduced in the kidneys of Ang II‐infused mice (p<0.05, results expressed as AU): miR‐22 (4,734 ± 1,327 vs. 2,144 ± 637), miR‐133a (34±5 vs. 22±2), miR‐1187 (619±227 vs. 140±66), miR‐574‐5p (501±193 vs. 148±53), and miR‐669f (110 ±43 vs. 25±13). In turn, the expression of 8 microRNAS was increased: miR‐155 (91±17 vs. 151±28), miR‐128 (145±15 vs. 196±4), miR‐183 (49±6 vs. 75±8), miR‐139‐5p (194±9 vs. 294±33), miR‐132 (84±5 vs. 187±39), miR‐200C (804±147 vs. 1,385±280), miR‐25 (895+89 vs. 1,223±103), and miR‐676 (54±6 vs. 98±11). In conclusion, these results demonstrate that there are significant changes in the microRNA expression profile of the mouse kidney during Ang II‐dependent hypertension. Supported by NIH grants P20RR017659, HL26371, and 1K99DK083455.