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The differentiation potential of rat bone marrow stem cells to cartilage and integrin antibody can blocking this course in vitro
Author(s) -
Deng Juan,
Jin Huan,
Liu Jian,
Liu Yongming
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.1030.7
Subject(s) - integrin , microbiology and biotechnology , chondrogenesis , extracellular matrix , chemistry , cellular differentiation , cartilage , in vitro , mesenchymal stem cell , cell , biology , anatomy , biochemistry , gene
Integrins mediate cell attachment to a variety of extracellular matrix proteins. These interactions play an important role in morphogenesis and differentiation. The mediating functions of integrins during chondrogenesis in vitro were investigated by using BMSCs of adult rat. This research have three groups:A,The cells were treated with anti‐β1‐ Integrin, dexamethasone, insulin, VitC .B,The cells were treated with TGF‐β3, dexamethasone, insulin, VitC .C,the control group .Cells were harvested after 7 and 14 days. The differentiation of the cells was investigated with bright‐field microscopy, the expression patterns of distinct proteins using immunohistochemistry and western blotting . the group A :The chondrocytes showed a strong proliferation. collagen I and II were downregulated; collagen II and Integrin‐β 1 was activated. But the blocking group could not happen,Integrin‐β1 were downregulated and the expression of collagen II was negative. These findings suggest that β1‐integrins play a crucial role in cartilage differentiation and point to a possible important cell‐matrix interaction in the induction of chondrogenesis .