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Optimizing the extraction and purification of maize‐produced Ag2 as a potential vaccine against Valley fever
Author(s) -
Kaur Kamaljeet,
Guzman Carlos,
Le Carmen,
Lagasca Dominique,
Esquerra Raymond
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.07447
Subject(s) - extraction (chemistry) , spore , protein subunit , chemistry , nitrogen , chromatography , downstream processing , microbiology and biotechnology , biology , biochemistry , organic chemistry , gene
Valley fever is an infection caused by inhaling spores of the fungal species Coccidioides . A proline rich protein, Ag2, from Coccidioides has been shown to induce an immune response against Valley fever. In this study, Ag2 was expressed in maize to be used as a candidate for a protein subunit vaccine. In order to achieve higher yields, we studied the impact of different detergents coupled with liquid nitrogen during the extraction process. The process was further optimized with downstream purification, resulting in a high‐yielding purified product. Our research showed that the grinding with liquid nitrogen in the presence of tween‐20 led to higher yields after purification.