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Elucidating the Protein‐Protein Interactions between Nontypeable Haemophilus Influenzae Outer Membrane Proteins Omp26 and Protein D
Author(s) -
Farquharson Kara Louise,
Jackson Niaya,
Cheng Susan Shi,
Kaur Ravinder,
Michel Lea Vacca
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.02607
Subject(s) - haemophilus influenzae , bacterial outer membrane , membrane protein , antibody , in vivo , in vitro , chemistry , microbiology and biotechnology , biology , biochemistry , immunology , membrane , escherichia coli , gene , antibiotics
Two leading protein vaccine candidates from Nontypeable Haemophilus Influenzae (NTHi) are outer membrane proteins Omp26 and Protein D. When administered individually, Omp26 and Protein D elicit an antibody response in mice; when combined, the Protein D antibody response is significantly suppressed. Here, we describe a biochemical approach toward elucidating the interactions between Omp26 and Protein D, which we propose contribute to the suppression of Protein D‐specific antibody production. Preliminary data from enzyme‐linked immunosorbent assays and other standard protein detection methods suggest that Protein D binds strongly to Omp26 in vitro and perhaps in vivo in mice.