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Effects of the Organophosphate Pesticide Chlorpyrifos on Muscle Architecture in 72 hour Zebrafish Embryos
Author(s) -
Willrich Robert C.,
Watanabe Taylor H.,
Fradinger Erica A.
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.644.18
Subject(s) - organophosphate , chlorpyrifos , zebrafish , embryo , cholinergic , acetylcholinesterase , biology , microbiology and biotechnology , pesticide , anatomy , toxicology , andrology , neuroscience , medicine , genetics , biochemistry , ecology , enzyme , gene
Chlorpyrifos (CPF) and other organophosphate pesticides (OP) are commonly used in the agricultural industries of the United States and developing countries. High concentrations of OP's in rivers and lakes have led to concerns for its negative effects on the wildlife and humans. These substances have been documented to act as neurotoxins that lead to the buildup of acetylcholinesterase in cholinergic neurons. This study was performed to determine the effects of CPF on the development of muscles for zebrafish embryos 72 hours post‐fertilization (hpf) in a calcium‐depleted medium. Using confocal microscopy of wholemount MF20 immuno‐labeled embryos, several morphological changes were observed. There was an increase in the length of muscle myomeres and width of individual muscle fibers in CPF exposed embryos. Additionally, some embryos showed strong muscle fiber disorganization. These observations show that CPF has a clear effect to muscle development and architecture possibly due to increased cholinergic stimulation during development. Support or Funding Information Department of Defense, ARO 66367‐RT‐REP; Keck Foundation Undergraduate Fellowship to R.C.W.; James Irvine Chair in Biological Sciences to E.A.F. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .