Open AccessIn vitroselection of DNA-cleaving deoxyribozyme with site-specific thymidine excision activity
Author(s) -
Mingqi Wang,
Huafan Zhang,
Wei Zhang,
You Xing Zhao,
Aneela Yasmeen,
Li Zhou,
XiaoQi Yu,
Zhuo Tang
Publication year - 2014
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/gku592
Subject(s) - deoxyribozyme , biology , deoxyribonucleotide , thymidine , dna , nucleotide , dna repair , nucleotide excision repair , biochemistry , genetics , microbiology and biotechnology , base excision repair , oligonucleotide , gene
Single-nucleotide polymorphisms, either inherited or due to spontaneous DNA damage, are associated with numerous diseases. Developing tools for site-specific nucleotide modification may one day provide a way to alter disease polymorphisms. Here, we describe the in vitro selection and characterization of a new deoxyribozyme called F-8, which catalyzes nucleotide excision specifically at thymidine. Cleavage by F-8 generates 3'- and 5'-phosphate ends recognized by DNA modifying enzymes, which repair the targeted deoxyribonucleotide while maintaining the integrity of the rest of the sequence. These results illustrate the potential of DNAzymes as tools for DNA manipulation.