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The genomes of thousands of organisms are being sequenced, often with accompanying sequences of cDNAs or ESTs. One of the great challenges in bioinformatics is to make these genomic sequences and genome annotations accessible in a user-friendly manner to general biologists to address interesting biological questions. We have created an open-access web service called WebGMAP (http://www.bioinfolab.org/software/webgmap) that seamlessly integrates cDNA-genome alignment tools, such as GMAP, with easy-to-use data visualization and mining tools. This web service is intended to facilitate community efforts in improving genome annotation, determining accurate gene structures and their variations, and exploring important biological processes such as alternative splicing and alternative polyadenylation. For routine sequence analysis, WebGMAP provides a web-based sequence viewer with many useful functions, including nucleotide positioning, six-frame translations, sequence reverse complementation, and imperfect motif detection and alignment. WebGMAP also provides users with the ability to sort, filter and search for individual cDNA sequences and cDNA-genome alignments. Our EST-Genome-Browser can display annotated gene structures and cDNA-genome alignments at scales from 100 to 50 000 nt. With its ability to highlight base differences between query cDNAs and the genome, our EST-Genome-Browser allows biologists to discover potential point or insertion-deletion variations from cDNA-genome alignments.

WebGMAP: a web service for mapping and aligning cDNA sequences to genomes