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Preparation of the two nonanucleotides, d(pG-G-A-G-G-A-G-A-A) and d(pT-T-C-T-G-T-T-G-A), and the octanucleotide, d(pT-G-G-C-C-C-A-T) is described by a rapid solid-phase method on a polyamide support. The syntheses were carried out using a modified solid-phase peptide synthesiser and required one day for each nucleotide addition. A microparticulate anion-exchanger, Partisil 10SAX, was used for the efficient fractionation of synthetic, base-protected oligonucleotides.

Rapid synthesis of oligodeoxyribonucleotides II1. Machine-aided solid-phase syntheses of two nonanucleotides and an octanucleotide

Rapid synthesis of oligodeoxyribonucleotides II¹. Machine-aided solid-phase syntheses of two nonanucleotides and an octanucleotide