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We have cloned and sequenced a Saccharomyces cerevisiae homolog of the mammalian ribosomal protein L9. The corresponding ORF, which is uninterrupted by introns, is 573 bp, encoding a protein of 191 amino acids with a calculated MW of 21.6 kDa. The gene, which we have named YL9A (Yeast L9A), is located on chromosome VII, immediately downstream of the ARO2 gene, encoding chorismate synthase (1). We have named this gene YL9A in order to distinguish it from a transcribed and presumably functional homolog, YL9B, which we have located by OFAGE and blot hybridisation on chromosome XVI. Gene disruption experiments demonstrate that the YL9A gene, although expressed as a 0.8 kb transcript, is non-essential, probably due to the presence of the homolog. In common with many yeast ribosomal protein genes the YL9A upstream region contains large poly (dAT) stretches and a putative RAP1 binding site centered at position —305 with respect to the translation start (2). In conjunction with two putative TATA elements located at positions 8 5 and 9 3 , these elements may be at least partially responsible for the production of the single mapped YL9A transcript species, which initiates at position -19 . The sequence analysis and the use of directional probes in Northern analysis demonstrate that the YL9A and ARO2 gene are convergently transcribed with only 224 bp between the stop codons of the two genes (Figure 1). Transcript end point mappings reveal a single 3' end of YL9A mRNA at position +671, showing that the 3' ends of the ARO2 (1) and YL9A mRNAs virtually abut, resulting in a mere 11 bp of intergenic space between the two transcribed regions.

Cloning and characterisation of a yeast homolog of the mammalian ribosomal protein L9