English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

The temperature-inducible expression vector pJLA5O3 (1) contains the c 1857-controlled lambda promoters PR/PL in tandem followed by the translation initiation region (TIR) of the E. coli atpE gene and convenient cloning sites. Transcription is terminated at the bacteriophage fd terminator (fdtt). The TIR region of the atpE gene has been suggested to be highly efficient in translation initiation (2). We have replaced the TIR region of pJLA503 with the 5' untranslated region, including the ribosome binding site (RBS) of the bacteriophage T7 gene 10 (3) from the expression vector pET3a (4) generating the temperature-inducible expression vector pJW2 (Fig. 1) which contains convenient unique cloning sites around the RBS {Ndel, Sphl, BamHl, EcoRl, Sail) and initiation codon (located within the Ndel restriction site) for gene insertion. We have found pJW2 much superior to pJLA503 for both temperature induced (42 °C) and nalidixic acid induced expression of a number of genes, including the genes coding for the mature form of porcine somatotropin (PST) (Fig. 2), precursor forms of PST, /3-galactosidase, j3glucuronidase and a-amylase. Recommended E. coli hosts include the protease-deficient host AR68 (5) and JA221 (6).

An efficient temperature-inducible vector incorporating the T7 gene10translation initiation leader region