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The SCE2£ gene was isolated by complementing a MAla cdc2 6 ura3 strain with a S. cerevisiae wild type genomic library in the vector pFLl (1). The SCD2 6 gene maps in the vicinity of CEC2£ (chromosome 6, right arm) (2) and complements the temperaturesensitive rrir:26 mutation (group H) (3) upon integrative gene duplication. The SCD2 6 gene is not essential for viability and encodes a globular hydrophilic 17 kD protein (151 amino acids, calculated pi 5.1) containing a central cluster of acidic residues. The codon usage indicates a low expression rate, and the predicted polypeptide sequence is not related to 5415 protein sequences compiled in the PIR data base. The SCD26 gene might encode a regulatory protein related to the still unidentified CDC26 gene product.

Nucleotide sequence of yeastSCD26, a dosage-dependent suppressor of the cell cycle mutationcdc26