English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Open

Presents the pdf analysis as premium feature

PDF Analysis

Insights

Presents the summarisation as premium feature

Summarisation

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Recently, low and high resolution restriction maps of the entire E. coli chromosome were constructed (1,2). This allows rapid physical mapping of genes on the chromosome. Efficient use of the high resolution restriction map awaits the availability of computerized version of the genomic map and a method for aligning restriction map data on it. Here, we describe a simple and efficient method for manually aligning such data. The high resolution physical map of E. coli (2) contains restriction map data for 8 enzymes. The graphical representation resembles a music score, parallel lines for each enzyme with vertical bars indicating cut sites. The restriction map data of the gene to be mapped should be graphically represented on a transparent film, exactly to the same format and scale. Physical mapping of a gene simply requires sliding the transparent film over the genomic restriction map. Inspection of the superimposed restriction maps easily reveals alignment (see Figure) and hence, the location of the gene on the chromosome. Visual alignment does not require precise restriction map alignment. The advantage of this method is that it allows an approximate pattern match to be recognized, and this may handle ambiguities arising from process in data or unanticipated genetic rearrangements. Recently, this method was applied to physically mapping the skp gene (3) encoding a 17 K DNA binding protein (4).

An efficient manual method for aligning DNA restriction map data on very large genomic restriction maps