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SOURCE/DESCRIPTION; A 4.5 kb (pMCT96.1) or a 3.7 kb (pMCT96.2) of Mspl fragment from cosmid MCT96 isolated by an oligonucleotide (GTGTGTGTGTGTGTGTGTGT) (1) was subcloned into the Accl site of pUC18. POLYMORPHISM: pMCT96.1 : Rsal identifies 3 systems of site polymorphisms; system 1: 4.7 kb (Rl) or 3.7 kb (R2); system 2: 3.0 (R3) kb or 2.8 kb (R4); system 3: 1.0 kb (R5) or 0.9 kb (R6) pMCT96.2 : Hinfl resolves a 4 allele VNTR polymorphism with bands between 1.5 kb and 3.0 kb. Mspl, TaqI, Rsal, PvuII and PstI also detect this polymorphism. HOT POLYMORPHIC FOR: none known CHROMOSOMAL LOCALIZATION: pMCT96.1 and pMCT96.2 have been assigned to chromosome 9q by multipoint linkage analysis(2) with loci (ABO, ABL, AK1, ORM) known to span this region(3). MENDELIAN INHERITANCE: Co-dominant segregation of the polymorphism was observed in 43 three generation families. PROBE AVAILABILITY: Freely available (contact Y.N.) or will be available from ATCC. OTHER COMMENTS: RFLPs were observed after competitive hybridization with total human DNA for pMCT96.2. For pMCT96.1, RFLPs were observed under normal hybridization and washing condition.

Isolation and mapping of a polymorphic DNA sequence (pMCT 96.1 and pMCT 96.2) on chromosome 9q (D9S14)