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A site of genomic insertion of the mouse retrotransposon LTR-IS/MuRRS was analysed. The comparison of the genomic and the cDNA clones indicates the insertion of the LTR-IS element into the 3' untranslated region of a mouse gene. The fact that the isolated cDNA clone ends with a poly A tail 20 nucleotides downstream from the LTR-IS AATAAA box and the result of the S1-nuclease mapping provides evidence that the 3' end of the mouse gene transcript was generated under the control of the LTR-IS polyadenylation signal.

Insertion of a retrotransposon within the 3' end of a mouse gene provides a new functional polyadenylation signal