English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

We have microinjected genomic DNA clones containing the Moloney murine leukemia virus (M-MuLV) proviral genome and flanking mouse sequences from Mov-3, Mov-7 and Mov-10 mice into Xenopus laevis oocytes and analyzed the virus-specific transcription and translation products. These mouse strains carry a proviral genome copy of M-MuLV in their germ line at different chromosomal positions and differ from each other with respect to expression of the proviral genome. We show here that the different M-MuLV proviral genome copies were transcribed into virus-specific RNA with similar efficiencies. Transcription of viral RNA initiated correctly at the viral promoter in the 5' LTR, whereas the promoter in the 3' LTR was used with a much lower frequency, if at all, for initiation of RNA synthesis. Most of the virus-specific transcripts were smaller than authentic M-MuLV mRNA and confined to the oocyte nucleus. Using immunoprecipitation, we were not able to detect virus-specific proteins after injection of proviral DNA, whereas after injection of a comparable amount of M-MuLV mRNA viral protein was readily detectable.

Transcription of cloned Moloney murine leukemia proviral DNA injected intoXenopus laevisoocytes