Open AccessVanadate inhibits Feo-mediated iron transport inVibrio cholerae
Author(s) -
Minsoo Shin,
Camilo Gómez-Garzón,
Shelley M. Payne
Publication year - 2021
Publication title -
metallomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 75
eISSN - 1756-591X
pISSN - 1756-5901
DOI - 10.1093/mtomcs/mfab059
Subject(s) - vibrio cholerae , vanadate , sodium orthovanadate , ferrous , sodium azide , atpase , atp hydrolysis , chemistry , biochemistry , microbiology and biotechnology , hydrolysis , biophysics , sodium , bacteria , biology , enzyme , phosphatase , genetics , organic chemistry
Iron is an essential element for Vibrio cholerae to survive, and Feo, the major bacterial system for ferrous iron transport, is important for growth of this pathogen in low-oxygen environments. To gain insight into its biochemical mechanism, we evaluated the effects of widely used ATPase inhibitors on the ATP hydrolysis activity of the N-terminal domain of V. cholerae FeoB. Our results showed that sodium orthovanadate and sodium azide effectively inhibit the catalytic activity of the N-terminal domain of V. cholerae FeoB. Further, sodium orthovanadate was the more effective inhibitor against V. cholerae ferrous iron transport in vivo. These results contribute to a more comprehensive biochemical understanding of Feo function, and shed light on designing effective inhibitors against bacterial FeoB proteins.