Open AccessAssisted Reproductive Techniques and Genetic Manipulation in the Common Marmoset
Author(s) -
Jung Eun Park,
Erika Sasaki
Publication year - 2020
Publication title -
ilar journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.129
H-Index - 75
eISSN - 1930-6180
pISSN - 1084-2020
DOI - 10.1093/ilar/ilab002
Subject(s) - biology , genetically modified organism , marmoset , genome editing , computational biology , zygote , in vitro fertilisation , transgene , genetics , gene , embryo , crispr , embryogenesis , paleontology
Genetic modification of nonhuman primate (NHP) zygotes is a useful method for the development of NHP models of human diseases. This review summarizes the recent advances in the development of assisted reproductive and genetic manipulation techniques in NHP, providing the basis for the generation of genetically modified NHP disease models. In this study, we review assisted reproductive techniques, including ovarian stimulation, in vitro maturation of oocytes, in vitro fertilization, embryo culture, embryo transfer, and intracytoplasmic sperm injection protocols in marmosets. Furthermore, we review genetic manipulation techniques, including transgenic strategies, target gene knock-out and knock-in using gene editing protocols, and newly developed gene-editing approaches that may potentially impact the production of genetically manipulated NHP models. We further discuss the progress of assisted reproductive and genetic manipulation techniques in NHP; future prospects on genetically modified NHP models for biomedical research are also highlighted.