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Nuclear factor 1 (NF1) affects accurate termination and multiple‐round transcription by human RNA polymerase III
Author(s) -
Wang Zhengxin,
Bai Lin,
Hsieh YngJu,
Roeder Robert G.
Publication year - 2000
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/19.24.6823
Subject(s) - rna polymerase ii , biology , library science , genetics , gene , promoter , computer science , gene expression
We have shown previously that the TFIIIC1/TFIIIC1′ fraction interacts specifically with the VA1 terminator regions to affect both termination and initiation/reinitiation of transcription by human RNA polymerase III. Here, we further purified the VA1 terminator‐binding factor to apparent homogeneity and found, by peptide sequence analysis, that it belongs to the NF1 protein family. NF1 interacts specifically with the NF1‐binding sites within the terminator regions of the VA1 gene and with two subunits (TFIIIC220 and TFIIIC110) of human TFIIIC2. Immunodepletion with anti‐NF1 antibodies dramatically decreases transcription from the VA1 template in nuclear extract, and mutation at the NF1‐binding site in the terminator region of the VA1 gene selectively affects multiple‐round transcription (reinitiation of transcription) and termination. In addition, NF1 acts in conjunction with TFIIIC to promote accurate termination by RNA polymerase III on a C‐tailed VA1 template.