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Cloning and characterization of p52, the fifth subunit of the core of the transcription/DNA repair factor TFIIH
Author(s) -
Marii JeanChristophe,
Roy Richard,
Vermeulen Wim,
Miniou Pierre,
Lutz Yves,
Weeda Geert,
Seroz Thierry,
Gomez Denise Molina,
Hoeijmakers Jan H. J.,
Egly JeanMarc
Publication year - 1997
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/16.5.1093
Subject(s) - transcription factor ii h , biology , cloning (programming) , genetics , transcription factor , transcription (linguistics) , microbiology and biotechnology , dna , computational biology , dna repair , gene , nucleotide excision repair , linguistics , philosophy , computer science , programming language
TFIIH is a multiprotein factor involved in transcription and DNA repair and is implicated in DNA repair/transcription deficiency disorders such as xeroderma pigmentosum, Cockayne syndrome and trichothiodystrophy. Eight out of the nine genes encoding the subunits forming TFIIH have already been cloned. We report here the identification, cDNA cloning and gene structure of the 52 kDa polypeptide and its homology with the yeast counterpart TFB2. This protein, along with p89/XPB, p62, p44 and p34, forms the core of TFIIH. Moreover, using in vitro reconstituted transcription and nucleotide excision repair (NER) assays and microinjection experiments, we demonstrate that p52 is directly involved in both transcription and DNA repair mechanisms in vitro and in vivo .