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A new Spectrophotometric flow injection method has been established for the determination of Cu (II) ions in analytical sample . The method is based on the reaction of Cu (II) ions with 1- (4-( 1- ( 2- hydroxyl phenyl amino) ethyl ) phenyl) diazenyl ) naphthalene-2- ol ( HPEDN) reagent in the presence of a Buffer solution has PH equal to 9 to form a red-water-soluble stable complex, that has a maximum absorbance at 500 nm . Beer’s law is obeyed in the range of 0.5-10 μg.ml-1 with a limit of detection (signal/noise=3) of 0.2213 μg.ml-1 and limit of quantification of 0.7377 μg.ml-1 and relative standard deviations of 0.65 %, The effect of chemical and physical parameters have been carefully considered and the proposed procedure was successfully applied to the determination of Cu (II) ions in analytical sample with a good precision and accuracy.

FI-Spectrophotometric determination of Cu (II) ions in analytical sample Via reaction with a new reagent HPEDN