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In this work, a simple method hydrazide-capturing technique followed by MALDI MS and MS/MS analysis for characterization of major pollen allergens of Olea europaea pollen was exploited. The strategy developed has different stages: devising of sample preparation protocol to sequester glycoproteins from a crude extract, and allowing the identification of antigenic peptides, glycosylation sites and modified glycans by MALDI MS and MS/MS.

Use of Galactose Oxidase in Hydrazide-Capturing Technique to Isolate N-linked Glycoallergens from Olive Tree Pollen