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Residual acidic slopes which are not covered by vegetation greatly increases the risk of soil erosion. In addition, low soil pH can bring numerous problems such as Al and Fe toxicity, land degradation issues and some problems related to vegetation. In this research, a series of electrokinetic bioremediation (EK-Bio) treatments using  Bacillus sphaericus, Bacillus subtilis  and  Pseudomonas putida  with a combination of Vetiver grass were performed in the laboratory. Investigations were conducted for 14 days and included the observation of changes in the soil pH and the mobilization of microorganism cells through an electrical gradient of 50 V/m under low pH. Based on the results obtained, this study has successfully proven that the pH of soil increases after going through electrokinetic bioremediation (EK-Bio). The treatment using  Bacillus sphaericus  increases the pH from 2.95 up to 4.80, followed by  Bacillus subtilis  with a value of 4.66. Based on the overall performance,  Bacillus sphaericus  show the highest number of bacterial cells in acidic soil with a value of 6.6 × 102 cfu/g, followed by  Bacillus subtilis  with a value of 5.7 × 102 cfu/g. In conclusion,  Bacillus sphaericus  and  Bacillus subtilis  show high survivability and is suitable to be used in the remediation of acidic soil.

Novel Technique to improve the pH of Acidic Barren Soil using Electrokinetic-bioremediation with the application of Vetiver Grass