English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Coronavirus disease-19 (COVID-19) pandemic caused millions of deaths and socio-economics damage worldwide. Corresponding to this, many studies on antiviral drugs exploration are rising to investigate the potential of drug compounds that can repress the replication of the SARS-CoV-2 virus by targeting its main protease named 3-chymotrypsin like protease (3CLpro). Without 3CLpro splicing, polyproteins of SARS-CoV-2 will not function to form new virions. We conducted an  in silico  thermodynamic study, and found several already known compounds from natural sources such as lovastatin, quinidine, and quinine were potential in inhibiting 3CLpro. However, most of the findings still need further wet-lab experiments to confirm their activity against it. To facilitate rapid screening of protease inhibitors, we aim to develop a screening kit for researchers with focus studies on herbal sources for COVID-19 treatments. Hence, the development of 3CLpro as the target of inhibition screening is essential. Our current work focused on the expression of the SARS-CoV-2 3CL Pro gene harbored by pET-32b(+) in  E. coli  BL21(DE3) and its purification. The recombinant  E.coli  were cultivated in LB media at 37 °C with 5 hours IPTG-induced phase followed by crude protein lysate extraction by sonication, and then Ni-NTA purification. A band of recombinant 3CLpro protein (33.8 kDa) detected by SDS PAGE and Western blotting after Ni-NTA purification was specific and clear, showing the right size of our protein interest. A final concentrated protein mixture obtained by 10-kDa membrane filtration displayed &gt;90% purity with a total protein of 2.497 mg mL −1 . Further functional assays and inhibitory tests with several natural compounds showed that 3CLpro functioned well as a drug target.

iop conference series. earth and environmental science

Production of recombinant SARS-CoV-2 3CL-protease: The key for the development of protease inhibitors screening kit in search of potential herb cure for COVID-19