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Lipase is one of the industrially important enzymes, however, the production needs costly substrate. To overcome the problem, we developed an effective medium formulation to produce lipase from indigenous lipolytic mould  Aspergillus aculeatus  Ms.11 using spent coffee ground. We observed the effect of additional glucose and olive oil, and the influence of water content on lipase production. The experiments were done using the Solid-State Fermentation (SSF) method for 7 days. The results show that optimum lipase production on substrates with additional glucose and olive oil observed on day 4 with lipase activity of 16.296 U/mL and lipase productivity of 150.32 U/g/day. The optimum water content from the results is 50%. The highest lipase activity obtained using the water content is 291.80 U/mL, while the highest lipase productivity is 106.32 U/g/day. The results showed that water content as well as the addition of glucose and olive oil, affects lipase productivity of  Aspergillus aculeatus  Ms.11 on spent coffee ground. Further studies to optimise the production condition are suggested.

The effect of water content and carbon source addition on lipase production from Aspergillus aculeatus Ms.11 using spent coffee ground