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Geodorum densiflorum  is an ornamental and medicinally important orchid. The medicinal metabolites are produced by endophytic fungi associated with orchid tissue, particularly flowers. However, there is no report of the endophytic fungi from the  G. densiflorum  flowers. This research aimed to study the endophytic fungi from different parts of  G. densiflorum  flower. Fungal isolation was carried out from sepal, petal, labium, stigma, and anther using surface sterilization method. The fungi were identified by combining morphological and molecular characteristics of ITS rDNA region. The results showed that each flower organ had different species of endophytic fungi. A total of seven species of endophytic fungi were obtained; four species were successfully identified by molecular identification and three species based on morphology. The four species, namely  Hypomontagnella barbarensis, Aspergillus oryzae, Curvularia pseudobranchyspora , and  Nigrospora chinensis , while the three species, namely  Gonatobotrys  sp.,  Humicola  sp.,  Aspergillus  section  Nigri . The labium inhabited by  Curvularia pseudobranchyspora, Nigrospora chinensis, Aspergillus  section  Nigri . Three species isolated from petals, namely  Hypomontagnella barbarensis, Gonatobotrys  sp., and  Aspergillus oryzae . The sepal is inhabited by  Humicola  sp. There were no endophytic fungi in stigma and anther. This indicates that each flower part is a unique habitat of endophytic fungi.

Flower endophytic fungi of Geodorum densiflorum endangered orchid