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Phosphates are important components in the fermentation medium for biosurfactant production. In the present study,  Ochrobactrum intermedium  strain XY-1 was used as the experimental strain, the effects of various phosphates used in the biosurfactants fermentation media was preliminarily investigated on cell growth, and surface tension, and emulsifying activity of fermentation broth. Under the same total phosphorus concentration of 0.035 mol/L, KH 2 PO 4  + Na 2 HPO 4 , KH 2 PO 4 , and Na 2 HPO 4  was respectively used as the phosphorus source of biosurfactant fermentation at 30 °C and 160 rpm. The results showed that the bacteria grew slowly and the maximum emulsifying activity of fermentation broth reached 52.95% in the fermentation with KH 2 PO 4  + Na 2 HPO 4  as phosphorus source, and larger bacterial growth and 35.9% maximum emulsifying activity exhibited in the fermentation with KH 2 PO 4 , smaller bacterial growth and 24.54% maximum emulsifying activity exhibited in the fermentation with Na 2 HPO 4 . The phosphate mixture of KH 2 PO 4  and Na 2 HPO 4  benefits the biosurfactant fermentation by limiting the growth of  Ochrobactrum intermedium  strain XY-1, which might be the earliest report on the phosphorous source of biosurfactant fermentation by  Ochrobactrum intermedium.

Effects of Various Phosphates on Biosurfactant Fermentation by Ochrobactrum Intermedium XY-1