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To substantiate the development of alternative agrarian and food technologies, the article presents the results of a study of the biological activity of potential probiotic cultures  Bacillus albus  and  Lactobacillus brevis  in the context of their further use as part of therapeutic and prophylactic additives to feed for various purposes. The biological activity of genuine strains of bacilli and lactobacilli was assessed by the effect of self-aggregation in the presence of polyvinylpyrrolidone nanowires obtained by the electrospinig method. The immobilized cells were cultured in an RTS-1C bioreactor with Reverse-Spin technology for 110 hours, followed by an assessment of the effect of immobilization by the kinetics of growth, pH of the culture medium and preservation of viability. As a result of a series of experiments, it was found that in the presence of PVP, growing cells of  Bacillus albus  and  Lactobacillus brevis  during fermentation acquire the ability to maintain a minimum growth rate and an optimal pH range for a long time period. In addition, in the presence of PVP, an increase in the number of viable cells of lactobacilli from 10 4  to 10 7  CFU/ml and bacilli from 10 4  to 10 6  CFU/ml was found in the reaction mixture. The data obtained are intended for further investigation of the phenomenon of self-aggregation of bacteria in the presence of polyvinylpyrrolidone nanowires and are considered promising for the development of new forms of probiotic products.

Biological activity of probiotics Bacillus albus and Lactobacillus brevis in the presence of polyvinylpyrrolidone nanofilaments