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As one of the most widespread raptors in Asia, Crested Serpent Eagle  (Spilornis cheela)  are genetically varied. However, due to the limited studies on genetic aspects, the genetic difference based its geographic distribution are difficult to define. A thorough phylogenetic analysis is needed to create a coherent taxonomic definition for the sake of conservation; starts with phylogenetic analysis based on  cytochrome-c oxydase subunit I (COI)  gene as the standardized genetic marker to define species. This research was aimed to resolve within species phylogenetic diversity and to explain the relationship between species based on  COI  gene. Total DNA was isolated from blood samples. The BLAST confirmed PCR amplified  COI  gene fragments were visualized on 0.8 agarose gel. Sequencing was carried out by FirstBase Laboratory Malaysia. Phylogenetic analysis was done using AMOVA and MEGA7 software in Neighbor Joining method with Kimura-2 parameter. Reconstructed phylogenetic tree confirmed the divergence between three samples and another  S. cheela  from other regions respective to their geographic distributions. There was 0,014 genetic distance between samples and  S. cheela  and 0,015 between  S. cheela  and other species among genus  Spilornis . This research also revealed that  S. cheela  and  S. holospilus  were not significantly varied.

iop conference series. earth and environmental science

A phylogenetic analysis of Crested Serpent Eagle (Spilornis cheela) based on cytochrome-c oxydase subunit I (COI): a stepping stone towards genetic conservation of raptors in Indonesia