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Feed efficiency, serum indices and selected intestinal bacterial population were evaluated in the Indonesian indigenous crossbred chickens (IICC) following treatments with the blends of butyric acid and  Bacillus subtilis  in the rations. A total of 200 of the IICC were randomly distributed to four dietary treatments including CNTRL (basal diet without additive), BTRT (basal diet added with 0.1% butyric acid), BACIL (basal diet added with 0.02%  B. subtilis ) and BTRBAC (basal diet added with the blends of 0.1% butyric acid and 0.02%  B. subtilis ). Weight gain, feed intake and feed efficiency of the IICC were recorded weekly. Blood was collected on week 8, and after which the chicks were slaughtered. Immediately, the digesta was obtained from the ileum and caecum of the IICC. Our findings showed that treatments improved ( P  0.05) the antioxidative status (serum malondialdehyde dan superoxide dismutase), antibody titer toward Newcastle disease vaccine, serum biochemical indices (cholesterol profile, total protein, albumin, globulin, uric acid) and the numbers of lactic acid bacteria and  Enterobacteriaceae  in the ileum and cecum of the IICC. In conclusion, dietary supplementation of butyric acid,  B. subtilis  or the combination of both improved the economic performance of the IICC.

iop conference series. earth and environmental science

Feed efficiency, serum indices and selected intestinal bacteria of the Indonesian indigenous crossbred chickens provided with the blends of butyric acid and Bacillus subtilis