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Bacteria load in Sungai Benut, Simpang Renggam Johor was assessed for its water quality index in this article. A total of 8 sampling stations at potential pollutant sources were analysed. These samples were tested for pH, temperature, biological oxygen demand (BOD), chemical oxygen demand (COD) and  Total coliform  (TC) as well as  Escherichia coli.  A river that has TC more than 5000 CFU/100 ml was categorized in Class IIA which need conventional treatment and sensitive to aquatic species. Average pH of Sungai Benut was recorded as acidic with pH 5.95 ± 0.94 with temperature in the range of 23.03 – 31.04 °C. Three stations included SP3, SP8 and SP9 recorded high bacterial load on the second sampling which are 12000, 12000 and 25000 CFU/100 mL for TC. The stations SP3, SP7 and SP10 recorded 3000, 20000 and 70000 CFU/100 mL of  E. coli  respectively. The Water Quality Index (WQI) of 11.73 – 33.95 was recorded for first sampling which indicate that Sungai Benut is polluted, need extensive treatment and prompt action from the authority. However, WQI of 29.20 – 64.25 was recorded on third sampling showing improving of water quality in Sungai Benut. Overall, Sungai Benut was fall into Class IV on first sampling which need extensive treatment and improve its quality on third sampling. Further monitoring is needed as it is still consider as slightly polluted.

Bacteria Load Assessment at Sungai Benut in Simpang Renggam, Johor