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Rhizobium  bacteria was known well as the bacteria which has potential on fixing nitrogen from the air and its widely used as biofertilizer. So far, the inoculants normally consist of  Rhizobium  bacteria which was packed in carrier material such as peat soil and applied to crop seed by mixing the seed with R hizobium  inoculant. This technique  Rhizobium  inoculant was not efficient anymore since much of bacteria cell was falling down, it did not completely sticked on the surface of seed. So this condition, resulted of un-effective function of the bacteria on running the nitrogen fixing process. To solve the problem, effort has been made to insert the bacteria inside the seed tissue using simple technology. This technology was developed for the target of symbiosis living between the bacteria and plants through optimal nitrogen fixing process. The purpose of this study is to confirm the presence of  Rhizobium  bacteria. That has been inserted into the soy bean seed using simple vacuum technology. The present of  Rhizobium  bacteria inside the seed tissue was observed from the preserved slices of seed tissue under amicroscope. The preserved slices of soybean seed tissue shown that the  Rhizobium  bacteria cell were able to reach the inside of seed tissue and fulfill the air space of palisade tissue.

Study of the presence of Rhizobium bacteria which were inserted into the cell tissue of soybean seed using vacuum technology