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Vibriosis caused by  Vibrio alginolyticus  infection is getting challenging to treat as the bacteria harbor the ability to form biofilm. One of the natural products that might be potentially applied to treat vibriosis through biofilm deformation is the leaves of  Moringa oleifera.  The objective of the study was to determine the effect of ethanol extracts of  M. oleifera  to inhibit  V. alginolyticus  biofilm formation in vitro. A microtiter plate biofilm assay (OD570nm) method was applied in this study. The treatments were the addition of ethanolic  Moringa  leaves extracts at different concentrations of 2%, 4%, 8% and 10%. The result of this study showed that  Moringa  leaves extract significantly inhibited the biofilm formation of  V. alginolyticus . The optimal condition to inhibit biofilm formation was at 10% concentration for 60 hours incubation at room temperature signifying the potential application of  Moringa  leaf extracts to treat biofilmmediated diseases in aquaculture.

Ethanolic extracts of Moringa oleifera leaves inhibit biofilm formation of Vibrio alginolyticus in vitro